- Cristobal Onetto (PhD student- University of Adelaide) travelled to the facilities of the Centre of Microscopy (University of Western Australia) to analyse winery wastewater samples using a NanoSIMS instrument.
- Established collaborations with Australia’s experts in NanoSIMS
- Two set of samples from different Australian wastewater treatment plants were analysed, one from a small winery in Yarra Valley, Victoria and another from a large winery in the Barossa Valley, South Australia.
- Basic principles in the operation of a NanoSIMS instrument were obtained.
Abstract
Winery wastewater (WWW) samples containing Glycogen Accumulating Organisms (GAO) were analysed using NanoSIMS (nanoscale secondary ion mass spectrometry). The main objective of this study was to observe the effect of nitrogen addition on the carbon metabolism of WWW bacteria. Incubations with different ratios of stable isotope labelled substrates were performed prior to analyses. Images showing the intracellular 15N and 13C enrichment of GAO were obtained. Significant differences were observed in the 15N and 13C levels of GAO incubated with different carbon:nitrogen ratios.
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Categories
Wastewater management
Winery management